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Granzyme D (GZMD)

Cat no: 153285

Granzyme D (GZMD)

Granzymes are serine proteases known mostly for their role in the induction of apoptosis. Granzymes A and B have been extensively studied, but relatively little is known about granzymes C to G and K to M. T cells, lymphohematopoietic stromal cells, and granulated metrial gland cells express granzyme D, but the function of granzyme D is unknown.\n\nApplications:\nSuitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:100-200. Recombinant Thyroglobulin (Ser2083~Ala2333) in loading buffer.\nUsage: 10ul per well when 3,3'-Diaminobenzidine(DAB) as the substrate.\n5ul per well when used in enhanced chemilumescent (ECL). Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.\nWestern Blot: 1:100-400\nImmunocytochemistry: 1:100-500 (formalin fixed cells)\nImmunohistochemistry: 1:100-500 (formalin fixed frozen sections)\nImmunohistochemistry: 1:50-200 (paraffin sections) \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

153285

Size

50ug

Applications

ELISA, ICC, IHC, WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

Infect Immun. 2013 Jun;81(6):2085-94. doi: 10.1128/IAI.00290-13. Epub 2013 Mar 25., R

Additional Info

Recognizes mouse GZMD in immunohistochemical staining and western blotting.

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