GRAP2 (GRB2 Related Adaptor Protein 2, GRB2 Like Protein, GADS, GRB2 Related Protein With Insert Domain, GRB2L, GRBLG, GrbX, Grf40, GRID, Growth Factor Receptor Binding Protein, GRPL, SH3-SH2-SH3 Adaptor Molecule)

This gene encodes a member of the GRB2/Sem5/Drk family. This member is an adaptor-like protein involved in leukocyte-specific protein-tyrosine kinase signaling. Like its related family member, GRB2-related adaptor protein (GRAP), this protein contains an SH2 domain flanked by two SH3 domains. This protein interacts with other proteins, such as GRB2-associated binding protein 1 (GAB1) and the SLP-76 leukocyte protein (LCP2), through its SH3 domains. Transcript variants utilizing alternative polyA sites exist.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications have not been tested. \n\nRecommended Dilutions: \nPeptide ELISA Titer: 1:32,000\nWestern Blot: 1-3ug/ml detects ~38kD band observed lysates of cell line Jurkat (calculated MW of 37.9kD according to NP_004801.1). In transfected HEK293 transiently expressing GRAP2 a band of approx. 40kD is observed. This band is not observed in the non-transfected HEK293. \nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G8959-13

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by peptide affinity chromatography.

Isotype

IgG

References

1.Qiu M, Hua S, Agrawal M, Li G, Cai J, Chan E, Zhou H, Luo Y, Liu M.Molecular cloning and expression of human grap-2, a novel leukocyte-specific SH2- and SH3-containing adaptor-like protein that binds to gab-1.Biochem Biophys Res Commun. 1998 Dec 18;253(2):443-7.

Additional Info

Recognizes human GRAP2. Species sequence homology: mouse, porcine.

Alternative Names

Mona, Grf40, RP3-370M22.1, growth factor receptor-bound protein 2-related adaptor protein 2, hematopoietic cell-associated adaptor protein GRPL, GRB2-related protein with insert domain, growth factor receptor-binding protein, SH3-SH2-SH3 adaptor molecule, GRB-2-like protein, GRB2-related adaptor protein 2, GRAP-2, GRBLG, GRB2L, Mona, GrbX, GRPL, GADS, P38, GRID, GRAP2