GRB 2 (Abundant SRC homology, Ash protein, Epidermal growth factor receptor binding protein, GRB2 adapter protein, Growth Factor Receptor Bound Protein 2, Growth factor receptor bound protein 3, HT027, SEM5, SH2/SH3 adapter GRB2)

Growth factor receptor bound protein 2 (GRB2), also known as Ash protein, is a 24kD protein that contains a central Src homology (SH2) domain flanked by two SH3 domains. GRB2 is believed to be a regulatory subunit of signaling molecules whose activity is modulated by receptor binding. GRB2 associates with activated (tyrosine phosphorylated) EGFR and PDGFR via its SH2 domain as well as IRS1, SHC, and LNK through SH2 and SH3 domains. The SH3 domain binds to SOS, a guanine nucleotide exchange factor for Ras proteins. Endocytosis of activated EGFR requires the interaction of GRB2 with the GTP binding protein dynamin, a factor essential to the formation of endocytotic vesicle. It is ubiquitously expressed and several isoforms are produced by alternative splicing. On SDS PAGE, GRB2 has an apparent molecular weight of 28kD.\n\nCellular Localization: Cytoplasmic (probable)\n\nApplications: \nSuitable for use in Western Blot and Immunohistochemistry (paraffin-embedded sections). Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G8960-06

Size

100ug

Applications

IHC, WB

Hosts

Goat

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in TBS, pH 7.2, 0.5% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

Lowenstein EJ et al. The SH2 and SH3 domain-containing protein GRB2 links receptor tyrosine kinases to ras signaling. Cell 70:431-42 (1992). <PUBMED:1322798>

Additional Info

Species Crossreactivity: Crossreacts with Human. Expected to crossreact with Rat and Mouse due to sequence homology.

Alternative Names

Abundant SRC homology, Ash protein, Epidermal growth factor receptor binding protein, GRB2 adapter protein, Growth Factor Receptor Bound Protein 2, Growth factor receptor bound protein 3, HT027, SEM5, SH2/SH3 adapter GRB2