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GRB10

Cat no: G8960-26

GRB10

GRB7 family adaptor proteins consist of GRB7, GRB10 and GRB14, which contain an N-terminal proline-rich SH3 binding domain, followed by PH, PBS and SH2 domains. Each member of the family has several splicing isoforms (1). It has been reported that GRB10 interacts with many receptor tyrosine kinases (RTKs) as well as downstream signal molecules such as Raf, Akt and Nedd4 (1,2). Although it was originally reported that GRB10 is exclusively phosphorylated at serine residues (3), recent research has shown that GRB10 is also phosphorylated at tyrosine 67 by Src kinase family members (4).\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nImmunoprecipitation: 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G8960-26

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.

P Type

Pab

Purity

Purified by protein A and peptide affinity chromatography.

Isotype

IgG

References

1. Han, D.C. et al. (2001) Oncogene 20, 6315

Additional Info

Recognizes endogenous levels of human total GRB10 protein independent of phosphorylation at 76kD. Does not crossreact with GRB7 or GRB14.

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