Grb14 (Growth Receptor-bound Protein 14)

Tankyrases (TRF1 interacting ankyrin-related ADP-ribose polymerase; human 1327 aa, renamed as TNKS-1/TANK1, chromosome 8, and its homolog TANK2), a modular protein with homology to ankyrin and poly(adenosine diphosphate-ribose) polymerase (PARP) has been cloned and localized to telomere. In addition to regulating the telomere and nuclear localization, TANKs also resides at the other subcellular compartments such as nuclear envelope, specifically on cytoplasmic fibers of nuclear pore complexes. TANK2 has also been shown to reside in the low-density microsomes and associate with the adapter protein, Grb14. The N-terminal 110aa of Grb14 and 10-19 ANK-repeats of TANK2 mediate this interaction. Grb14 (mouse/rat 538aa, human 540aa, chromosome 2) is a member of Grb7 (Growth receptor bound proteins) family that includes Grb7, and Grb10. Grb family is characterized by the presence of a conserved, short non-catalytic SH2 domain (Src homology region 2) that binds to peptide sequences containing phopshotyrosine. Many intracellular targets of receptor tyrosine kinases contain 1 or more SH2 domains. Grb14 is widely distributed in many tissues with an abundance in testis, ovary, heart, liver, skeletal muscle, kidney and pancreas. TANKs may play a role vesicle trafficking and in cytoplasmic signal transduction pathways via the Grg14.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 1:10,000-50,000. Control peptide (Cat #G8960-30L)\n an be used to coat ELISA plates at 1ug/ml.\nWestern Blot: 1:1000-1:5000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G8960-30D

Size

50ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Mouse, Rat

Form

Supplied as a liquid in PBS, 0.05% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Kasus-Jacobi, A., et al., JBC 273: 26,026 (1998). 2. Reilly, J.F., et al., JBC 275: 7771 (2000). 3. Daly, R.J., et al., JBC 271: 12,502 (1996). 4. Dong, L.Q., et al., JBC 272: 29,104 (1997). 5. Lyons, R.J., et al., JBC 276: 17,172 (2001).

Additional Info

Recognizes rat Growth Receptor-bound Protein 14. No significant sequence homology exist with other Grb7 family members.