Grb2 (Growth Factor Receptor-Bound Protein 2, ASH, Abundant Src Homology)

Grb2 is an adaptor protein which has no enzymatic activity; however, Grb2 plays an important role in signaling cascades by physically linking other proteins through its phosphotyrosine-binding SH2 domain and its proline-binding SH3 domain. Grb2 associates with the guanine nucleotide exchange factor Sos via its SH3 domain. In response to cell stimulation with growth factors, growth factor receptors are autophosphorylated on cytoplasmic tyrosine residues. Grb2

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SPECIFICATIONS

Catalog Number

G8960-02

Size

100ug

Applications

IF, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Xen/Amph

Form

Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 15mM sodium azide

P Type

Mab

Purity

Purified from ascites by Protein G affinity chromatography.

Isotype

IgG1,k

References

Boulikas, T. (1995) Phosphorylation of transcription factors and control of the cell cycle. Crit. Rev. Eukar. Gene Expr. 5(1):1-77.\nCarraway, K.L., and C.A. Carraway (1995) Signaling, mitogenesis and the cytoskeleton; where the action is. BioEssays 17(2):171-175.\nChardin, P., D. Cussac, S. Maignan, and A. Ducruiz (1995) The Grb2 adaptor. FEBS Lett. 369(1):47-51.\nDownward, J. (1994) The Grb2/Sem-5 adaptor protein. FEBS Lett. 338(2):113-117.\nSchlessinger, J. (1994) SH2/SH3 signaling proteins. Curr. Opin. Genetics Dev. \n4(1):25-30.\nScott, J.D. and T. Pawson (2000) Cell communication: The inside story. Sci. Am. June 2000:72-79.

Additional Info

Recognizes a protein with Mr24-25kD, identified as Grb2 (growth-factor receptor-bound protein 2), also known as ASH (abundant Src homology). Grb2 deletion studies indicate that the epitope for this antibody maps to amino acid residues 54-62. No crossreactivity with other SH2/SH3 signaling intermediates. Species Reactivity: Human, bovine, mouse, rat and Xenopus.