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Grb2 (Growth Factor Receptor-Bound Protein 2, ASH, Abundant Src Homology)

Cat no: G8960-03A

Grb2 (Growth Factor Receptor-Bound Protein 2, ASH, Abundant Src Homology)

GRB-2 (growth factor receptor-bound protein 2), an adaptor protein involved in signal \ntransduction, contains a central SH2 domain flanked by two SH3 domains. The SH2 domain \nbinds to phosphotyrosine residues in RTKs such as PDGF and EGF, non-RTKs such as \nBcr/Abl and FAK, and docking proteins such as FRS-2 and Gab1. The SH3 domains \nassociate with proline rich motifs on the guanine nucleotide releasing factor, Sos, stimulating \nGTP binding to Ras, which in turn activates MAPK and other signaling pathways. GRB-2 \nalso participates in the endocytosis of EGFR through its recruitment of Cbl. Tyrosine \nphosphorylation of GRB-2 SH3 domains reduces binding to Sos and negatively regulates \ndownstream signaling pathways including Ras, JNK and MAPK. \n \nApplications:\nWestern Blot: An antibody concentration of 1.0ug/ml is recommended. \n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G8960-03A

Size

100ug

Applications

WB

Hosts

Goat

Reactivities

Hum, Mouse, Rat

Form

Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Detects human, mouse and rat GRB-2.

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