GRB7, Growth Factor Receptor-Bound Protein 7, N-Terminus

The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with epidermal growth factor receptor (EGFR) and ephrin receptors. The protein plays a role in the integrin signaling pathway and cell migration by binding with focal adhesion kinase (FAK). Alternative splicing results in multiple transcript variants encoding different isoforms, although the full-length natures of only two of the variants have been determined to date.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications have not been tested. \n\nRecommended Dilutions: \nPeptide ELISA Titer: 1:32,000\nWestern Blot: 1-3ug/ml detects ~60kD band observed in lysates of cell line A431 (calculated MW of 59.7kD according to NP_005301.2). In transfected HEK293 transiently expressing GRB7 a band of approx. 60kD is observed. This band is not observed in the non-transfected HEK293.\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G8960-20B

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by peptide affinity chromatography.

Isotype

IgG

References

1.Margolis B, Silvennoinen O, Comoglio F, Roonprapunt C, Skolnik E, Ullrich A, Schlessinger J.\nHigh-efficiency expression/cloning of epidermal growth factor-receptor-binding proteins with Src homology 2 domains.Proc Natl Acad Sci U S A. 1992 Oct 1;89(19):8894-8

Additional Info

Recognizes human GRB7.

Alternative Names

OTTHUMP00000164352, growth factor receptor-bound protein 7, GRB7