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Green Fluorescent Protein (GFP)

Cat no: 029791

Green Fluorescent Protein (GFP)

Since the molecular cloning of GFP cDNA and demonstration of GFP as a functional transgene, GFP has become a powerful tool with exciting applications in developmental, cell and molecular biology. GFP fluorescence is not species specific and can be expressed in bacteria, yeast, plant and mammalian cells. GFP can also fuse with proteins of interest without interfering significantly with their assembly and function.\n\nApplications:\nSuitable for use in Immunofluorescence, Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence: 10-20ug/ml\nWestern Blot: 0.5-4ug/ml\nImmunoprecipitation: 2-8ug/ml\nImmunohistochemistry: 10-20ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

029791

Size

100ug

Applications

IF, IHC, IP, WB

Hosts

Mouse

Form

Supplied as a liquid in PBS, pH 7.2, 0.5% BSA, 0.01% thimerosal, 30% glycerol.

P Type

Mab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG1

Additional Info

Recognizes wild-type GFP and its derivatives in all species.

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