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Green Fluorescent Protein (GFP)

Cat no: 168229

Green Fluorescent Protein (GFP)

Green fluorescent protein is a 27kD protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. GFP is ideal for Cell Biology, Neuroscience and Cancer research.\n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:24,000-1:100,000\nWestern Blot: 1:500-1:5000\nImmunohistochemistry: 1:200-1:3000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

168229

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Rabbit

Form

Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

wan W-H, Navarro-Sanchez E, Dumortier H, Decossas M, Vachon H, et al. (2008) Dermal-Type Macrophages Expressing CD209/DC-SIGN Show Inherent Resistance to Dengue Virus Growth. PLoS Negl Trop Dis 2(10): e311. doi:10.1371/journal.pntd.0000311

Additional Info

Recognizes GFP.

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