Green Fluorescent Protein (GFP) (FITC)

Green Fluorescence Protein (GFP) is a 27kD protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.\n\nApplications: \nSuitable for use in ELISA, Flow Cytometry, Immunofluorescence and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1:2000-1:10,000\nImmunofluorescence: 1:500\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

G8965-04E1

Size

50ug

Applications

ELISA, FC, IF, WB

Hosts

Rabbit

Form

Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.01% sodium azide. Labeled with FITC.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP and EGFP. Species Crossreactivity: Minimum crossreactivity to human, mouse and rat serum proteins.

Alternative Names

Green Fluorescent Protein