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Green Fluorescent Protein (GFP) (FITC)

Green Fluorescent Protein (GFP) (FITC)

Cat no: G8965-04E2

Supplier: United States Biological
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GFP is a 27kD monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (395nm)and emits green light (508nm) in the absence of additional proteins, substrates, or co-factors. Intact, native GFP protein is required for fluorescence.The GFP chromophore results from the cyclization and oxidation of the sequence Ser65-Tyr66-Gly67. GFP fluorescence is stable, species independent, and suitable for a variety of applications. Applications: Suitable for use in ELISA, Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence: Tested on prokaryotic (E. coli) and eukaroytic (CHO cells) expression systems. Detected GRP containing inserts. Immunohistochemistry: Frozen sections. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: G8965-04E2
Reactivities: Goat
Hosts: Goat
Applications: ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry
Size: 100ul
Form: 0.02M Potassium phosphate, 0.15M sodium chloride, [pH7.2]
P type: Pab
Isotype: IgG
Purity: Purified
Alternative names: Green Fluorescent Protein
References: 1. The TH & Feltkamp TE Conjugation of fluorescein isothiocyanate to antibodies. I. Experiments on the conditions of conjugation. Immunology 18:865-73 (1970).
Additional info: Recognizes GFP and its variants. Species Crossreactivity: Does not react with human, mouse and rat proteins.

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