Green Fluorescent Protein (GFP) (FITC)

Anti-GFP assayed in a sandwich ELISA is well suited to titrate GFP in solution using either form of the antibody as the capture or detection antibodies. The detection antibody is typically conjugated to biotin and complexed with streptavidin-HRP. Fluorochrome conjugated G8965-12A was assayed by immunofluorescence microscopy on prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and was shown to detect GFP containing inserts. Significant amplification of signal was detected using fluorochrome conjugated G8965-12A relative to the fluorescence of GFP alone. \n\nApplications: \nSuitable for use in ELISA and Immunofluorescence. Other applications not tested. \n\nRecommended Dilution: \nImmunofluorescence 1:500-1:2500\nELISA: Sandwich or capture\nOptimal dilution determined by the researcher. \n\nAbsorption Wavelength: 495nm\n\nEmission Wavelength: 528nm\n\nFluorochrome/Protein Ratio: \n2.9 moles FITC per mole of Goat IgG\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

G8965-12A

Size

1mg

Applications

ELISA, IF, WB

Hosts

Goat

Form

Supplied as a lyophilized powder from PBS, pH 7.2,10mg/ml BSA, (IgG and Protease free), 0.01% sodium azide. Labeled with fluorescein isothiocyanate (FITC). Reconstitute with 1ml sterile ddH2O.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes Green Fluorescent Protein (GFP). Assayed by ELISA for direct binding of antigen, recognizing wild type, recombinant and enhanced forms of GFP. Assay by IEP resulted in a single precipitin arc against anti-goat serum, anti-Fluorescein and purified and partially purified green fluorescent protein (Aequorea victoria) serum. No reaction was observed against human, mouse and rat serum proteins.