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Green Fluorescent Protein (GFP)

Cat no: G8965-01

Green Fluorescent Protein (GFP)

GFP is a 27kD monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (395nm) and emits green light (508nm) in the absence of additional proteins, substrates or co-factors. Intact, native GFP protein is required for fluorescence. The GFP chromophore results from the cyclization and oxidation of the sequence Ser65-Tyr66-Gly67. GFP fluorescence is stable, species independent and suitable for a variety of applications.\n\nApplications: \nSuitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications have not been tested.\n\nRecommended Dilution: \nWestern Blot: 1:500-1:1000\nELISA: 1:1000-1:10,000\nImmunohistochemistry: 1:1000.\nMouse brain neural cells (1:1000) RT, overnight using secondary antibody anti-rat IgG-Cy3 (1:300) RT, 1hr. Data courtesy of Yoshihiro Yoshyihara RIKEN Brain Science Institute.\nOptimal dilutions to be determined by the researcher.\n\nHybridoma: Mouse GF200\n\nSource: Ascites\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G8965-01

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Mouse

Form

Supplied as a liquid in PBS, 0.02% sodium azide before the addition of glycerol to 40%.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1,k

References

1. Prasher, D.C., et al. (1992) Gene 111:229-233 (1982). 2.

Additional Info

Reacts with Green Fluorescent Protein (GFP) and crossreacts with Blue Fluorescent Protein (BFP).

Read more on Supplier website

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info
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