Green Fluorescent Protein (GFP)

Green fluorescent protein (GFP), a 27kD protein derived from the jellyfish Aequorea victoria, emits green light (emission peak at a wavelenth of 509nm) when excited by blue light (excitation peak at a wavelenth of 395nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein-protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP is used to measure single cell metastasis and successful proliferation of stem cells. In these ways, GFP technology has contributed to a greater understanding of cellular biology and biochemistry.\n\nApplications:\nSuitable for use in Immunocytochemistry and Western Blot. Other applications have not been tested.\n\nRecommended Dilution:\nELISA: 1:2000-1:10,000\nWestern Blot: 1:1000-1:5000. Detects a band of ~28kD. using colorimetric detection (Peroxidase/TMB), with higher dilution possible using more sensitive detection methods.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G8965-02B

Size

100ug

Applications

ELISA, WB

Hosts

Rabbit

Form

Supplied as a liquid in TBS, pH 7.2, , 0.01% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography. >99% by SDS-PAGE & HPLC

References

Understanding, improving and using green fluorescent proteins: A.B. Cubitt, et al.; TIBS 20, 448 (1995)\nChalfie, M., Y. Tu, G. Euskirchen, W.W. Ward and D.C. Prasher. 1994. Green-fluorescent protein as a marker for gene expression. Science 263:802-805.\n

Additional Info

Recognizes GFP and GFP fusion proteins from both native and recombinant sources. This anti-GFP recognizes all variants of A. victoria GFP induced in transformed organisms, including all vertebrate species tested.