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Green Fluorescent Protein (GFP)

Cat no: G8965-06

Green Fluorescent Protein (GFP)

Green fluorescent protein (GFP) is a 29kD fluorescent protein isolated from the jellyfish Aequorea victoria. Upon exposure to blue light (488nm), GFP strongly fluoresces (Emission max: 507nm). GFP can be fused to proteins without significantly interfering with their assembly and function, making GFP a powerful tool for monitoring gene expression and protein localization in living cells\n\nApplications: \nSuitable for use in Western Blot and Immunocytochemistry. Other applications have not been tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-2ug/ml detects GFP from 50ug of rat brain lysate containing 25ng of purified GFP. Also detected a GFP-fusion protein from a transfected E. coli cell lysate. Rat brain lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with G8965-06 (1ug/ml). Proteins were visualized using a rabbit anti-chicken secondary antibody conjugated to HRP* and a chemiluminescence detection system. \nImmunocytochemistry: Reported to show positive immunostaining in cells transfected with an expression vector encoding GFP. \nOptimal dilutions to be determined by the researcher.\n\nRecommended Secondary Antibodies: \nI1906 IgY, Chicken Pab Rb x Ch \nI1906-10 IgY, Chicken (HRP) Rb x Ch \nI1906-12 IgY, Chicken (FITC) Rb x Ch \nI1906-14 IgY, Chicken (AP) Rb x Ch\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G8965-06

Size

200ug

Applications

ICC, WB

Hosts

Ch/Bird

Form

Supplied as a liquid in 100mM Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide, 30% glycerol.

P Type

Pab

Purity

Purified by ammonium sulfate precipitation

Isotype

IgY

References

Gerdes, H. H. and Kaether, C., FEBS Letters 389: 44

Additional Info

Recognizes green fluorescent protein (GFP), Mr 30kD, and GFP-fusion proteins. Species Crossreactivity: Vertebrates

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