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Green Fluorescent Protein (GFP)

Cat no: G8965-10D

Green Fluorescent Protein (GFP)

The jellyfish Aequorea victoria contains green fluorescent protein (GFP) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights into the physiological activities of living cells.\n\nApplication(s): Western Blot. \n\nRecommended Dilution: Dot blot titers were performed on nitrocellulose membranes using 0.7ug of GFP at a 1:2,500,000 dilution, 1:2000 dilution of alkaline phosphatase conjugated goat anti-rabbit IgG.

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SPECIFICATIONS

Catalog Number

G8965-10D

Size

50ul

Applications

WB

Hosts

Rabbit

Form

Supplied as a liquid in 25mM Tris HCl pH 7.75, 50mM sodium chloride, 0.02% sodium azide

P Type

Pab

Purity

Purified by affinity chromatography.

Isotype

IgG

Additional Info

Reacts against all variants of recombinant Aequorea GFP.

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