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Green Fluorescent Protein, Recombinant (GFP)

Cat no: G8965-10E

Green Fluorescent Protein, Recombinant (GFP)

Full-length Green Fluorescent Protein with N-terminal HIS-tag (6X), recombinant, expressed in E. coli. \n\nGFP is a 27kD monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (395nm) and emits green light (508nm) in the absence of additional proteins, substrates, or co-factors. Intact, native GFP protein is required for fluorescence. The GFP chromophore results from the cyclization and oxidation of the sequence Ser65-Tyr66-Gly67. GFP fluorescence is stable, species independent, and suitable for a variety of applications.\n\nAccession Number: AAA27722\n\nApplications: \nSuitable for use as a standard for protein gels and Western Blots (~28kD). Also suitable for calibration of ?uorometers and microinjection into cells and tissues. Other applications not tested.\n\nRecommended Dilutions:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G8965-10E

Size

50ug

Applications

WB

Form

Supplied as a liquid in PBS, 20% glycerol.

Purity

Purified using Ni-NTA agarose (~70%).

References

1.

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Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info
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