Green Fluorescent Protein, Recombinant, Jellyfish

Supplier:

United States Biological

Cat no: G8965-10F

Green Fluorescent Protein, Recombinant, Jellyfish

GFP is a 27kD monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (395nm) and emits green light (508nm) in the absence of additional proteins, substrates, or co-factors. Intact, native GFP protein is required for fluorescence. The GFP chromophore results from the cyclization and oxidation of the sequence Ser65-Tyr66-Gly67. GFP fluorescence is stable, species independent, and suitable for a variety of applications. \n\nSource:\nRecombinant Jellyfish GFP, full length, with C-terminal HIS tag, expressed in E. coli . \n\nMolecular Weight: 28.6kD\n\nAbsorbance:\n395 and 470nm, emits green fluorescence at 509nm. \n\nApplications: \nSuitable for use as a quantitative control for GFP expression studies using GFP reporter \nvectors. It can also be used as a standardon protein gels and western immunoblots or for calibration of Fluorometers, flow cytometers and FACS machines. Other applications not tested.\n\nRecommended Dilution: \nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.

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SPECIFICATIONS

Catalog Number

G8965-10F

Size

200ug

Form

Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM NaCl, 0.05% Tween-20, 50% glycerol.

Purity

(same/more than)90%

Read more on Supplier website

SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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