A novel green fluorescent U2OS cell line has been developed through stable transfection with tGFP. This cell line expresses green fluorescent protein gene sequences as free cytoplasmatic proteins. tGFP-U2OS Cell line is stably-transfected clonal cell line that is ready to use in cell-based assay applications. This stably transfected clonal cell line provides consistent levels of expression, which helps simplify the interpretation of results. This cell line is intended to be used as in vitro model for research studies.