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GREM1, Recombinant, Human, aa25-184, His-Tag (Gremlin-1 Isoform 1, CKTSF1B1, DAND2, DRM, GREMLIN, IHG-2, PIG2)

Cat no: 138659

GREM1, Recombinant, Human, aa25-184, His-Tag (Gremlin-1 Isoform 1, CKTSF1B1, DAND2, DRM, GREMLIN, IHG-2, PIG2)

GREM1 is a member of the BMP (bone morphogenic protein) antagonist family. Like BMPs, BMP antagonists contain cystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which this protein belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted glycosylated protein is likely due to its direct binding to BMP proteins. As an antagonist of BMP, this protein may play a role in regulating organogenesis, body patterning, and tissue differentiation. In mouse, this protein has been shown to relay the sonic hedgehog (SHH) signal from the polarizing region to the apical ectodermal ridge during limb bud outgrowth. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. \n\nSource:\nRecombinant protein corresponding to aa25-184 from human GREM1 at N-terminal, fused to His-tag expressed in E.coli. \n\nMolecular Weight: \n~20.7kD (183aa)\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MGSKKKGSQG AIPPPDKAQH NDSEQTQSPQ QPGSRNRGRG QGRGTAMPGE EVLESSQEAL HVTERKYLKR DWCKTQPLKQ TIHEEGCNSR TIINRFCYGQ CNSFYIPRHI RKEEGSFQSC SFCKPKKFTT MMVTLNCPEL QPPTKKKRVT RVKQCRCISI DLD\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

138659

Size

100ug

Form

Supplied as a liquid in tris-HCl buffer, pH 8.0, 0.4M urea, 10% glycerol.

Purity

~90% (SDS-PAGE)

References

1. Helena Stabile, Stefania Mitola, et al. (2007). Blood 109:5 1834-1840.

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