Glutamate receptors constitute the principal excitatory neurotransmitter receptors in brain. Two classes of glutamate receptors consist of: Ionotropic receptors, and metabotropic receptors. Ionotropic glutamate receptors are oligomeric complexes of various subunits (GluR1-7, NMDA1-3, KAI-2), which comprise of ligand-gated calcium channels. Metabotropic glutamate receptors are G-protein coupled receptors, once stimulated it can activate phopholipases or adenylylcyclase, depending on the neuron.
Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunohistochemistry: 1:50 for 10min at RT
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10min followed by cooling at RT for 20min.
Optimal dilutions to be determined by the researcher.
Positive Control:
Brain
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.