Griffonia (Bandeiraea) simplicifolia GS I-B4

The seeds of Griffonia simlicifolia contain four distinct lectins. Two of these lectins, referred to as GS-I and GS-II, have been well characterized with regard to binding specificity and structure. GS-II is specific for terminal, non-reducing alpha or beta linked N-acetyl-D-glucosamine. This lectin is not blood group specific, unlike GS-I. GS-II is also a tetramer. It is composed of identical subunits. Each subunit has a single carbohydrate binding site for GlcNAc. GS-II is the only lectin isolated that is specific for only terminal GIcNAc residue. The subterminal saccharide does play an important role in lectin binding.\n\nBiological Activity:\n20-30ug/ml is required to agglutinate fresh type B blood cells. Lectin activity against all blood types increases after neuraminidase treatment of cells.\n\nCarbohydrate Specificity: a-Galactose\nInhibitory Carbohydrate: Gal a(1,3) > Methyl-a-galactoside . Galactose > Methyl-b-galactoside > N-acetylgalactosamine.\n\nNote: Griffonia simlicifolia (GS-1) exists as a mixture of 5 isolectins. Each isolectin is a tetramer consisting of different amounts of subunits termed A and B. The number after the subunit designation indicates the quantity of each subunit associated with the tetramer form. The GS-1B4 isolectin has four B-subunits. It has a specificiety for type B ethrocytes.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Reconstitute to nominal volume by adding sterile buffer of choice, aliquot and store at -20 degrees C. May be stored at 4 degrees C for short-term only. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G8967-60

Size

1mg

Form

Supplied as a lyophilized powder from PBS, pH 7.2, 0.5mM calcium chloride. Reconstitute with buffer of choice. Note: Calcium is required for binding. 0.5mM calcium is the maximum concentration in buffer that will not form a white precipitiate.

References

1. Eckhardt, A.E., et al. Cancer Res. 42:2977-2979 (1986). 2. Judd, W.J., et al., Transfusion (Philadelphia). 18:274-280 (1978). 3. Maddox, D.E., et al., PNAS. 79:166-170 (1982). 4. Murphy, L.A., and Goldstein, I.J., J. Biol. Chem. 252:4739-4742 (1977). 5. Shanker Iyer, P.N., et al. Arch. Biochem. Biophys. 177:330-333 (1976).

Alternative Names

Bandeiraea simplicifolia