Griffonia simplicifolia Lectin I Isolectin B4 (Bandeiraea simplicifolia) (GSL I

GSL I-B4 isolectin contains only the B subunits and has been reported to be useful as a marker for endothelial cells from nonprimates such as mouse, rat, rabbit, and goat. This biotinylated lectin conjugate is prepared from affinity-purified lectin and is optimally labeled with biotin. Essentially free of inactive lectin conjugate and containing no free biotin, this biotinylated lectin provides an ideal intermediate for examining glycoconjugates using the Biotin-Avidin System. First the biotin-labeled lectin is added, followed by an ABC Reagent, Avidin D conjugate, or streptavidin derivative. Another possible application is in the isolation of lymphokines and other products of mitogenic stimulation.\n\nApplications: \nSuitable for use in ELISA, Western Blot and Histochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 5-20 ug/ml\nHistochemistry (paraffin or frozen sections): 5-20 ug/ml\nWestern Blot: 5-20 ug/ml\nOptimal dilutions to be determined by the researcher.\n\nInhibiting Sugar: \n500mM galactose or 100mM raffinose\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in above buffer.

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SPECIFICATIONS

Catalog Number

G8967-93B

Size

500ug

Applications

ELISA, IHC, WB

Conjugates

Biotin

Form

Supplied as a liquid in 10mM HEPES, pH 7.5, 0.15M sodium chloride, 0.08% sodium azide, 0.1mM Ca++. Labeled with Biotin.

References

1. Alroy, et. al., Histochemistry 86,603-607 (1987). Laitinen, Histochem. J. 19,225-234 (1987). 2. Laitinen, et. al., J. Histochem. Cytochem. 35,55-65 (1987). 3. Christie & Thomson, J. Histochem. Cytochem. 37,1303-1304 (1989). 4. Augustin-Voss, et. al., Vet. Pathology 27,103-109 (1990).