GRIP1 (Glucocorticoid Receptor Interacting Protein 1, NCOA2 TIF2, Transcriptional Intermediary Factor 2)

Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand-dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate. Glucocorticoid receptor interacting protein-1 (GRIP 1), or Transcription Intermediary Factor 2 (TIF 2), is a member of a family of transcriptional coactivator proteins which includes steroid receptor coactivator-1 (SRC 1) and Amplified in breast cancer-1 (AIB 1).GRIP1 has been shown to interact and stimulate transcriptional activity of the retinoic acid, retinoid X, vitamin D, mineralocorticoid, glucocorticoid, thyroid hormone, androgen, estrogen, progesterone, and peroxisome proliferator activated receptors. GRIP 1 also contains several copies of the conserved LXXLL/LLXXL motif which has been demonstrated to be critical to receptor-coactivator interactions.\n\nPositive Control: CHO cells\n\nApplications: \nSuitable for use in Immunohistochemistry (Fr). Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G8970-07

Size

100ug

Applications

IHC

Hosts

Rabbit

Reactivities

Hum

Form

PBS, 1 mg/ml BSA, 0.05% sodium azide

P Type

Pab

Purity

Purified

Isotype

IgG

References

Hong H et al. An additional region of coactivator GRIP1 required for interaction with the hormone-binding domains of a subset of nuclear receptors. J Biol Chem 274:3496-502 (1999). <PUBMED:9920895>Schmidt S et al. Multiple receptor interaction domains of GRIP1 function in synergy. Nucleic Acids Res 26:1191-7 (1998). <PUBMED:9469826>Hong H et al. GRIP1, a transcriptional coactivator for the AF-2 transactivation domain of steroid, thyroid, retinoid, and vitamin D receptors. Mol Cell Biol 17:2735-44 (1997). <PUBMED:9111344>

Additional Info

Species Crossreactivity: Crossreacts with Human and Hamster.Not yet tested in other species.

Alternative Names

GRIP1, glutamate receptor interacting protein 1, HGNC:18708, GRIP