GRK 2,3 (betaARK 1,2)

Applications:\nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilutions:\nImmunoprecipitation: This antibody can be used to immunoprecipitate GRK2/3 from transfected cells. \nWestern Blot: 0.1-1ug/ml. Detects GRK2/3 in RIPA lysates from mouse 3T3 fibroblasts. Previous lots of this antibody detected GRK2/3 in bovine brain cytosol preparation, rat L6 myoblasts, human A431 carcinoma and human HeLa cell RIPA lysates. 3T3 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with G8972 (0.1ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. \n\nRecommended secondary antibodies: \nI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-14C: IgG, Fc (HRP) Pab Gt xMo \nI1904-19C: IgG, F(ab

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SPECIFICATIONS

Catalog Number

G8972

Size

100ug

Applications

IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Ch/Bird, Prc, Rabbit

Form

Supplied in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide., 40% glycerol.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a,k

References

1. DebBurman, S.K., et al., J. Biol. Chem. 271: 22552-25564. 2, 1996. 2. Chuang, T.T., et al., J. Biol. Chem. 271: 28691-2869, 1996. 3. Application References: Daaka, Y., et al., Proc. Natl. Acad. Sci. USA 94: 2180-2185, 1997. 4. Freedman, N.J., et al., J. Biol. Chem. 272: 17734-17743,1997. 5. ppermann, M., et al., Proc. Natl. Acad. Sci. USA 93: 7649-7654, 1996.

Additional Info

Recognizes human GRK2/3 at ~80 kD. Additional bands at 85 and 50 kDa are also detected when using higher concentrations of the antibody. Epitope maps to aa483-485. Species Crossreactivity: rat, mouse, chicken, bovine, porcine and rabbit.