GRK3, CT (G Protein Coupled Receptor Kinase 3, Beta-adrenergic Receptor Kinase 2, ADRBK2, Beta-ARK-2, BARK2) (AP)

The beta-adrenergic receptor kinase specifically phosphorylates the agonist-occupied form of the beta-adrenergic and related G protein-coupled receptors. Overall, the ADRBK2 enzyme, also known as GRK3, has 85% amino acid similarity with ADRBK1, with the protein kinase catalytic domain having 95% similarity. The ADRBK2 mRNA is approximately 8 kilobases with a distribution similar to that of ADRBK1. These data suggest the existence of a family of receptor kinases which may serve broadly to regulate receptor function.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:1,000 \nWestern Blot: 1:100-1:500\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G8972-05-AP

Size

200ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to Alkaline Phosphatase.

P Type

Pab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1.Calabrese, G., et al., Genomics 23(1):286-288 (1994). 2.Parruti, G., et al., Biochem. Biophys. Res. Commun. 190(2):475-481 (1993). 3.Benovic, J.L., et al., J. Biol. Chem. 266(23):14939-14946 (1991).

Additional Info

Recognizes human GRK3. Species Crossreactivity: mouse, primate.

Alternative Names

Adrenergic, beta, Receptor Kinase 2, EC=2.7.11.15