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GRK5 (G Protein-Coupled Receptor Kinase 5, G Protein-coupled Receptor Kinase GRK5, GPRK5)

Cat no: G8972-21J

GRK5 (G Protein-Coupled Receptor Kinase 5, G Protein-coupled Receptor Kinase GRK5, GPRK5)

GRK5 is a 590aa protein belonging to the G protein-coupled receptor kinase family and consisting of AGC-kinase C-terminal, protein kinase and RGS domains. It phosphorylates the activated forms of G protein-coupled receptors, thus initiating their deactivation. It also phosphorylates the human substance P receptor and plays a role in regulating the motility of polymorphonuclear leukocytes (PMNs). It contains a membrane binding motif consisting of a group of hydrophobic amino acids that mediates its plasma membrane (PM) localization. GRK5 is expressed in heart, placenta, lung, skeletal muscle, brain, liver, pancreas and kidney.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-4ug/ml\nImmunohistochemistry (paraffin): 20ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G8972-21J

Size

100ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov

Form

Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Arraes, SM. et al. Blood. 108:2906-2913 (2006). 2. Thiyagarajan, MM. et al. J. Biol. Chem. 279:17989-17995 (2004) 3. Warabi, K. et al. FEBS. Lett. 521:140-144 (2002).

Additional Info

Recognizes human GRK5. Species Crossreactivity: bovine, mouse, rat. Species sequence homology: chicken, pufferfish, zebrafish.

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