GRK5 (G Protein-Coupled Receptor Kinase 5, G Protein-coupled Receptor Kinase GRK5, GPRK5)

G protein-coupled receptor kinases (GRKs) play an important role in phosphorylating and regulating the activity of a variety of G protein-coupled receptors. GRK5 is a Ser/Thr protein kinase and a member of the guanine nucleotide-binding protein GRK subfamily. Itphosphorylates the activated forms of GRKs which initiates their deactivation. It has also been shown to play a role in regulating the motility of polymorphonuclear leukocytes (PMNs). Desensitization of GRKs regulates the number of PMNs, as well as their motility and ability to stop upon contact with\npathogens or target cells. MIP2 induces GRK2 and GRK5 expression in PMNs through PI3KG \nsignaling. However, lipopolysaccharide (LPS), acting through TLR4, and mediated through MEK1/2, transcriptionally downregulates expression of GRK2 and GRK5 in response to MIP2. This decreases chemokine receptor desensitization and markedly augments PMN migration. Thus, LPS-activated TLR4 signaling regulates PMN migration by modulating the expression of chemokine receptors in a GRK2- and GRK5-dependent manner. \n\nApplications: \nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (Formalin fixed paraffin embedded): 7ug/ml \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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