GRO alpha, Recombinant, Human (KC)

Three human MGSA/GRO genes encode 3 highly related chemokines, MGSA/GRO alpha, -beta and -gamma. All 3 MGSA/GRO proteins bind to the same receptors, but with differing affinities, and stimulate a number of biological responses including chemotaxis, angiogenesis, and growth regulation. Melanoma growth stimulatory activity/gro alpha (MGSA), a member of the alpha-chemokine family, is produced by a variety of dermal and epidermal cells and can act in a paracrine and autocrine fashion.\n\nDescription: \nRecombinant Human GRO-alpha produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 73 amino acids and having a molecular mass of 7811 Dalton. Recombinant Human GRO-alpha is purified by proprietary chromatographic techniques.\n\nAmino Acid Sequence: \nThe sequence of the first five N-terminal amino acids was determined and was found to be Ala-Ser-Val-Ala-Thr.\n\nBiological Activity: \nRecombinant Melanoma growth stimulatory activity is fully biologically active when compared to standard. The Biological activity is calculated by its ability to chemoattract human neutrophils cells using a conc. of 10.0-100.0 ng/ml.\n\nProtein Content: \nProtein quantitation was carried out by two independent methods:\n1. UV spectroscopy at 280 nm .\n2. Analysis by RP-HPLC, using a calibrated solution of MGSA as a Reference Standard.\n\nSolubility: \nIt is recommended to reconstitute the lyophilized GRO-alpha in sterile 18MOmega-cm H2O not less than 100microg/ml, which can then be further diluted to other aqueous solutions.\n\nStability: \nLyophilized MGSA/GRO-a although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution MGSA/GRO-alpha should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

G8975-16

Size

5ug

Form

Supplied as a lyophilized powder without additives.

Purity

(same/more than) 98% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.

References

1. Melanoma growth stimulatory activity in primary malignant melanoma: prognostic significance.\n\nMod Pathol 2002 May;15(5):532-7\n\n2. A role for poly(ADP-ribose) polymerase in the transcriptional regulation of the melanoma growth stimulatory activity (CXCL1) gene expression.\n\nJ Biol Chem 2001 Mar 23;276(12):9366-74\n\n3. Nuclear factor-kappa B activation by the CXC chemokine melanoma growth-stimulatory activity/growth-regulated protein involves the MEKK1/p38 mitogen-activated protein kinase pathway.\n\nJ Biol Chem 2001 Feb 2;276(5):3650-9\n\n4. Melanoma growth stimulatory activity (MGSA/GRO-alpha) chemokine genes incorporated into an African green monkey simian cytomegalovirus-derived stealth virus.\n\nExp Mol Pathol 1999 Apr;66(1):15-8\n\n5. Enhanced tumor-forming capacity for immortalized melanocytes expressing melanoma growth stimulatory activity/growth-regulated cytokine beta and g amma proteins.\n\nInt J Cancer 1997 Sep 26;73(1):94 -103\n\n6. Role of melanoma growth stimulatory activity (MGSA/gro) on keratinocyte function in wound healing.\n\nArch Dermatol Res 1997 Mar;289(4):204-12