Gross Cystic Disease Fluid Protein 15 (GCDFP-15)

Gross Cystic Disease Fluid Protein-15 (GCDFP-15, BRST-2) is a unique 15 kD monomer protein found in the cystic fluid of gross cystic disease which is a common premenopausal disorder. The protein is also known under different names in different body fluids such as gp17 in seminal plasma. It has been reported that GCDFP-15 is a ligand of CD4 and is a potent inhibitor of T-cell apoptosis induced by sequential CD4/T-cell receptor triggering. The cells within the body that produce GCDFP-15 appear to be restricted primarily to those with apocrine function.\n\nApplications: \nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry: frozen and formalin-fixed, paraffin-embedded tissue sections\nEpitope Retrieval: Not required\nEnzyme Digestion: Not required\nOptimal dilutions to be determined by the researcher.\n\nPositive Control Tissue:\nBreast cancer\n\nExpected Staining Pattern:\nCytoplasmic\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n

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SPECIFICATIONS

Catalog Number

G2023-30

Size

1ml

Applications

IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.1% sodium azide before the addition of glycerol to 40%.

P Type

Mab

Purity

Ascites

Isotype

IgG2a

References

1. Haagensen, C.D. et al. 1986. Disease of the breast. 3rd ed. PP250-267. W.B. Saunders Co. Philadelphia, PA\n2. Caputo, E. et al. 2000. A novel aspartyl proteinase from apocrine epithelia and breast tumors. J Biol Chem 275(11):7935-7941.\n3. Viacava, P. et al. 1998. Spectrum of GCDFP-15 expression in human fetal and adult normal tissues. Vichows Arch 432(3);255-\n260.\n4. Mazoujian, G. et al. 1983. Immunohistochemistry of a breast gross cystic disease fluid protein (GCDFP-15) : A marker of apocrine\nepithelium and breast carcinomas with apocrine features. Am J Pathol 110:105-111.\n5. Wick, M.R.et al. 1989. Gross Cystic Disease Fluid Protein-15 as a marker for breast cancer: Immunohistochemical analysis of\n690 human neoplasms and comparison with Alpha-lactalbumin. Hum Pathol 20(3): 281-187.\n6. Clark,J.W. et al. The potential role for prolectin-inducible protein (PIP) as a marker of human breast cancer micromatastasis. Br J\nCancer 81(6):1002-1008.\n7. Lagendijk, J.H. et al. 1999. Immunohistochemical differentiation between primary adenocarcinomas of the ovary and ovarian\nmetastases of colonic and breast origin. Comparison between a statistical and an intuitive approach. J Clin Pathol 52 (4): 283-290\n8. Caputo, E. et al. 1998. Differential antibody reactivity and CD4 binding of the mammary tumor marker protein GCDFP-15 from\nbreast cyst and its counterparts from exocrine epithlia. Int J Cancer 78(1): 76-85.\n9. Hall, R.E. et al. 1998. Prostate-specific antigen and gross cystic disease fluid protein-15 are co-expressed in androgen receptorpositive\nbreast tumors. Br. J Cancer 78(3):360-365.\n10. Kaufmann,O. et al. Immunohistochemical differentiation of metastatic breast carcinoma from metastatic adenocarcinomas of other\ncommon primary sites. Histopathol 29(3):233-240.\n11. Mazoujian, G. et al. 1984. Extramammary Paget

Additional Info

Recognizes human Gross Cystic Disease Fluid Protein 15 (GCDFP-15)