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Growth and Differentiation Factor 5 (GDF5)

Cat no: G8989-45A

Growth and Differentiation Factor 5 (GDF5)

Growth Differentiation Factor 5 (GDF5) is a member of the bone morphogenetic protein (BMP) subfamily of the TGF-beta superfamily. It is synthesized as a large precursor protein that consists of an N-terminal 19aa signal sequence, a 362aa pro region and a 130aa C-terminal mature peptide (aa376-495). Homodimeric GDF5 activity is mediated by receptor complexes containing type I (BMPR-IB) and type II (BMPR-II or Activin R-II) serine/threonine kinase receptors. GDF5 has multiple functions including the regulation of myogenesis, chondrogenesis, bone morphogenesis and neuronal differentiation and survival.\n\nApplications: \nSuitable for use in ELISA and Neutralization. Other applications have not been tested. \n\nRecommended Dilutions: \nDirect ELISA: 0.5-1.0ug/ml. The detection limit for recombinant mouse GDF-5 is ~10ng/well.\nNeutralization (ND50): ~2-10ug/ml in the presence of 3ug/ml of recombinant mouse GDF5, using the ATDC5 cell line. The exact concentration of antibody required to neutralize recombinant mouse GDF5 activity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity studied. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G8989-45A

Size

500ug

Applications

ELISA

Hosts

Rat

Reactivities

Mouse

Form

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.

P Type

Mab

Purity

Purified by Protein G affinity chromatography. Endotoxin: < 0.1EU/ug (LAL).

Isotype

IgG2b

Additional Info

Recognizes mouse GDF5. Does not crossreact with recombinant mouse GDF1, GDF3 or GDF8.

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