Growth Associated Protein 43 (GAP-43)

GAP-43 or growth associated protein 43 is a nervous system-specific phosphoprotein and a major component of motile growth cones which form the leading edge of the elongating axon. The membrane skeleton of the growth cone is responsible for transducing extracellular guidance cues into morphological alterations mediated by the polymerization/de-polymerization of actin resulting in directional changes in axon growth. GAP-43 is closely associated with the membrane skeleton and has been implicated in regulating interactions between cytoskeletal components and the plasma membrane. Interestingly, axonal transport of GAP-43 occurs only in neurons that are extending axons during development or regeneration after injury; however, this transport does not occur in axons that do not normally regenerate such as those in the mammalian central nervous system. These results suggest that the in vivo function of GAP-43 may be closely linked to axon growth and regeneration.\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nELISA (Immunogen): 0.1-1ug/ml \nWestern Blot: 1ug/ml \nImmunohistochemistry (Frozen and paraffin): 5ug/ml \nOptimal dilutions to be determined by the researcher.\n\nWB Positive Controls:\nCell lysates derived from mouse and rat brain.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G8990-10H

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Ch/Bird, Rabbit

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2a,k

References

1. Meiri, K.F., et al., J. Cell Biol. 112: 991-1005 (1991). 2. Karns, L.R., et al., Science 236: 597-600 (1987). 3. Basi, G.S., et al., Cell 49: 785-791 (1987). 4. Meiri, K.F., et al., Proc. Natl. Acad. Sci. 83: 3537-3541 (1986). 5. Tanaka, E., and Sabry, J., Cell 83: 171-176 (1995). 6. Meiri, K.F. and Gordon-Weeks, P.R., J. Neurosci. 10: 256-265 (1990). 7. Skene, J.H.P., Ann. Rev. Neurosci. 12: 127-156 (1989). 8. Coggins, P., and Zwiers, H., J. Neurochem. 53: 1895-1901 (1989). 9. Apel, E.D., et al., Biochemistry 29: 2330-2335 (1990). 10. Alexander, K., et al., J. Biol. Chem. 262: 6108-6113 (1987). 11. Chapman, E., et al., J. BIol. Chem. 266: 207-213 (1991). 12. Wang, K., and Roufogalis, B., Biochem. J. 262: 693-706 (1989). 13. Apel, E.D., Storm, D., Perspect. Dev. Neurobiol. 1: 3-11 (1992). 14. Dent, E.W. and Meiri, K.F., J. Neurobiol. 8: 1037-1053 (1992). 15. He, Q., et al., J. Neurosciences 17: 3515-3524 (1997). 16. Meiri, K.F., et al., J. Neurobiology 29: 213-232 (1995).

Additional Info

Recognizes rat GAP-43. Reacts with both phosphorylated and unphosphorylated forms of the GAP-43 protein. \nSpecies Crossreactivity: chicken, human, mouse, rabbit and snake.