Growth Hormone 2, Recombinant, Human, aa27-217 (GH2, GHL, GHV)

Growth Hormone 2 (GH2), also known as Growth Hormone Variant (GH-V) and Placental Growth Hormone (PGH), is a secreted 22kD glycoprotein in the Placental Lactogen/Prolactin peptide hormone family. GH2 shares considerable structural and functional similarity with the pituitary-derived Growth Hormone (GH) (1-3). Mouse and rat orthologs of GH2 have not been identified, but human GH2 is bioactive in mice as well as rats (4, 5). Alternative splicing of human GH2 generates a 20kD isoform with a deletion of amino acids 32-46 and isoforms with substituted C-terminal regions (3, 6). GH2 is secreted by placental syncytiotrophoblasts into the maternal circulation beginning early in gestation and rising throughout pregnancy, as the level of circulating GH concommitantly decreases (1, 3, 7). GH2 is also present at lower concentrations in the fetal circulation and amniotic fluid (8-10). It is elevated in the amniotic fluid of Down syndrome pregnancies, and maternal and fetal circulating levels may be either elevated or depressed during pre-eclampsia (10-12). Like GH, GH2 exerts its activities through the Growth Hormone Receptor (GHR) (13). It promotes trophoblast invasion of the uterine wall and the secretion of maternal IGF-I (14, 15). Maternal serum levels of GH2 positively correlate with IGF-I during the third trimester and with fetal birthweight (7, 16). GH2 induces maternal insulin resistance and exerts both somitogenic and anti-lipogenic activities (4, 5, 13). The 20kD isoform of GH2 also binds GHR and shows full anti-lipogenic activity, reduced somitogenic activity, and reduced diabetogenic activity (5, 13).\n\nSource:\nRecombinant protein corresponding to aa27-217 from human Growth Hormone 2, expressed in E. coli.\n\nMolecular Weight: \n~20kD\n\nEndotoxin:\n<1.0EU/1ug (LAL method).\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

145799

Size

25ug

Form

Supplied as a lyophilized powder in HCl, BSA. Reconstitute in 4mM HCl (>0.1% human or BSA).

Purity

~95% (SDS

References

1. McIntyre, H.D. et al. (2009) Curr. Diabetes Rev. 5:185. 2. Igout, A. et al. (1988) Arch. Int. Physiol. Biochim. 96:63. 3. Cooke, N.E. et al. (1988) J. Biol. Chem. 263:9001. 4. Barbour, L.A. et al. (2002) Am. J. Obstet. Gynecol. 186:512. 5. Vickers, M.H. et al. (2009) Am. J. Physiol. Endocrinol. Metab. 297:E629. 6. Boguszewski, C.L. et al. (1988) J. Clin. Endocrinol. Metab. 83:2878. 7. Fuglsang, J. et al. (2003) J. Clin. Endocrinol. 88:4355. 8. Higgins, M.F. et al. (2012) PLoS ONE 7:e29164. 9. Mittal, P. et al. (2008) Growth Horm. IGF Res. 18:174. 10. Mittal, P. et al. (2007) J. Matern. Fetal Neonatal Med. 20:651. 11. Sifakis, S. et al. (2009) Growth Horm. IGF Res. 19:121. 12. Mannik, J. et al. (2012) Mol. Cell. Endocrinol. 355:180. 13. Solomon, G. et al. (2006) Growth Horm. IGF Res. 16:297. 14. Lacroix, M.C. et al. (2005) Endocrinology 146:2434. 15. Caufriez, A. et al. (1993) Am. J. Physiol. 265:E572. 16. Mannik, J. et al. (2010) J. Clin. Endocrinol. Metab. 95:2433.