Growth Hormone Binding Protein, Human, Recombinant (GHR, GHBP, GH receptor, Somatotropin receptor)

GHBP is a transmembrane receptor for growth hormone. Binding of growth hormone to the receptor leads to receptor dimerization and the activation of an intra- and intercellular signal transduction pathway leading to growth. A common alternate allele of this gene, called GHRd3, lacks exon three and has been well-characterized. Mutations in this gene have been associated with Laron syndrome, also known as the growth hormone insensitivity syndrome (GHIS), a disorder characterized by short stature. Other splice variants, including one encoding a soluble form of the protein (GHRtr), have been observed but have not been thoroughly characterized.\n\nGrowth Hormone Binding Protein Human Recombinant produced in E. coli is a single, non-glycosylated, polypeptide chain containing 237 amino acids and having a molecular mass of 28,107.01D. GHR is purified by proprietary chromatographic techniques.\n\nGene Name: GHR\n\nProtein synonyms/aliases: Growth hormone receptor precursor (GH receptor) (Somatotropinreceptor) \n\nPrecursor's functional components:\nGrowth hormone binding protein (GH binding protein) (GHBP) (Serum binding protein) \n\nSource: \nEscherichia coli\n\nSolubility:\nVery soluble in water and most aqueous buffers below and above the isoelectric point. It is recommended to reconstitute the lyophilized GHBP in sterile 18MOmega-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. \n\nBiological Activity:\nGHBP is fully biologically active as evidenced by its ability of forming 2:1 complex with HGH.\n\nProtein content:\nProtein quantitation was carried out by two independent methods: \n1. UV spectroscopy at 280 nm using the absorbency value of 2.6 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). \n2. Analysis by RP-HPLC, using a calibrated solution of GHBP as a Reference Standard.\n\nAmino acid sequence: \nThe sequence of the first five N-terminal amino acids was determined and was found to be Ala-Phe-Ser-Gly-Ser, conforming to the sequence of native human GHBP. \n\nDimers and Aggregates: \n(same/less than) 1% as determined by silver-stained SDS-PAGE gel analysis. \n\nEndotoxin:\n<1ng/ug\n\nStability:\nLyophilized GHBP although stable at room temperature for 3 weeks, should be stored desiccated below -20 degrees C. Upon reconstitution GHBP should be stored at 4 degrees C between 2-7 days and for future use below 20 degrees C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid freeze-thaw cycles.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

G9000-13

Size

100ug

Form

GHBP was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3.

Purity

(same/more than) 98.0% as determined by RP-HPLC, Anion-exchange FPLC. Analysis by reducing and non-reducing SDS-PAGE Silver Stained.

References

1. Insulin-like growth factors, insulin-like growth factor-binding proteins, insulin-like growth factor-binding protein-3 protease, and growth hormone-binding protein in lipodystrophic human immunodeficiency virus-infected patients. Haugaard SB, Andersen O, Orskov H, Flyvbjerg A, Metabolism 2004 Dec;53(12):1565-73\n2. The gender differences in growth hormone-binding protein and leptin persist in 80-year-old men and women and is not caused by sex hormones. Bulow B, Ahren B, Dehlin O, Samuelsson G, Clin Endocrinol (Oxf) 2003 Oct;59(4):482-6\n3. Levels of plasma insulin-like growth factor I (IGF I), IGF II, IGF binding proteins, type 1 IGF receptor and growth hormone binding protein in community-dwelling elderly subjects with no malnutrition and no inflammation. Raynaud-Simon A, J Nutr Health Aging 2003;7(4):267-73\n4. Growth hormone binding protein. The soluble growth hormone receptor. Baumann G, Minerva Endocrinol 2002 Dec;27(4):265-76\n5. The growth hormone-binding protein is a location-dependent cytokine receptor transcriptional enhancer. Graichen R, Sandstedt J, Isaksson OG, J Biol Chem 2003 Feb 21;278(8):6346-54\n6. Growth hormone binding protein in cirrhosis: normal or decreased? Moller S, Fisker S, Henriksen JH, J Hepatol 2002 Nov;37(5):702-3