Growth Hormone BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human Growth Hormone in cell culture supernates, serum and plasma (heparin, EDTA).\n\nGrowth hormone (GH) is synthesized by acidophilic or somatotropic cells of the anterior pituitary gland. Human growth hormone has a molecular mass of 22,005 and contains 191 amino acid residues with 2 disulfide bridges. The human genes for growth hormone (GH), has been located on chromosome 17 in humans. GH replacement of adults with acquired GH deficiency (GHD) results in body composition changes including increases in lean mass and bone mineral density. However, the effects of long-term GH therapy on cognitive function are largely unknown, and there are conflicting data regarding quality of life. The standard product used in this kit is recombinant human Growth Hormone, consisting of 192 amino acids with the molecular mass of 22kD.\n\nAssay Range:\n15.6pg/ml-1000pg/ml\n\nSensitivity:\n< 2 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nB1A4G6\n\nEntrez Gene ID:\n2688\n\nKit Components:\n96-well plate pre-coated with anti-Growth Hormone, Human\nLyophilized recombinant Growth Hormone, Human standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

143859

Size

96Tests

References

1. Baum, H. B. A.; Katznelson, L.; Sherman, J. C.; Biller, B. M. K.; Hayden, D. L.; Schoenfeld, D. A.; Cannistraro, K. E.; Klibanski, A. : Effects of physiological growth hormone (GH) therapy on cognition and quality of life in patients with adult-onset GH deficiency. J. Clin. Endocr. Metab. 83: 3184-3189, 1998\n2. Niall, H. D.; Hogan, M. L.; Sauer, R.; Rosenblum, I. Y.; Greenwood, F. C. : \nSequence of pituitary and placental lactogenic and growth hormones: evolution from a primordial peptide by gene reduplication. Proc. Nat. Acad. Sci. 68: 866-869, 1971. \n3. Owerbach, D.; Rutter, W. J.; Martial, J. A.; Baxter, J. D.; Shows, T. B. : Genes for growth hormone, chorionic somatomammotropin and growth hormone-like genes on chromosome 17 in humans. Science 209: 289-292, 1980. \n