Growth Hormone, Pituitary, Recombinant, Human, 20kD (hGH)

Recombinant Human GH-20K is a single, non-glycosylated, polypeptide chain containing 177 amino acids and having a molecular mass of 20322 Dalton. \n\nSequence:\nThe sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Thr-Gly-Ser. \n\nDimers and Aggregates:\n(same/less than) 1% as determined by silver-stained SDS-PAGE gel analysis. \n\nBiological Activity:\nrHuGH-20K is fully biologically active when compared to World Health Organization (WHO) reference standard. \n\nEndotoxin:\n(same/less than) 0.1ng/ug (IEU/ug) of GH-20K. \n\nProtein Content:\nProtein quantitation was carried out by two independent methods: \n 1. UV spectroscopy at 280 nm using the absorbency value of 0.73 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). \n 2. Analysis by RP-HPLC, using a calibrated solution of GH-20K as a Reference Standard. \n\nReconstitution:\nReconstitute and dilute the lyophilized GH-20K in 0.4% NaHCO3 adjusted to pH 10, which can then be further diluted to other aqueous solutions. It is recommended when diluting to below 10ug/ml that the dilution solution will contain carrier protein (BSA or other) to avoid non-specific absorption of the hGH to plastic tubes. \n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 0.4% NaHCO3 and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G9000-17

Size

100ug

Form

Supplied as a lyophilized powder in 0.0045mM NaHCO3, pH 10.

Purity

(same/more than) 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.

References

1. Metabolic effects of 20-kilodalton human growth hormone (20K-hGH) for adults with growth hormone deficiency: results of an exploratory uncontrolled multicenter clinical trial of 20K-hGH. Hayakawa M, Shimazaki Y, Kato Y, Irie M, J Clin Endocrinol Metab 2004 Apr;89( 4):1562-71 2. Diabetogenic activity of 20 kDa human growth hormone (20K-hGH) and 22K-hGH in rats. Takahashi S, Shiga Y, Hayakawa M, Growth Horm IGF Res 2001 Apr;11(2):110-6 3. Effects of 22K or 20K human growth hormone on lipolysis, leptin production in adipocytes in the presence and absence of human growth hormone binding protein. Asada N, Takahashi Y, Horm Res 2000;54(4):203-7 4. Twenty-kilodalton human growth hormone (20K hGH) secretion from growth hormone-secreting pituitary adenoma cells in vitro. Murakami Y, Mori T, Kurosaki M, Endocr J 2000 Oct;47(5):563-8 5. Effects of octreotide infusion, surgery and estrogen on suppression of height increase and 20K growth hormone ratio in a girl with gigantism due to a growth hormone-secreting macroadenoma. Minagawa M, Yasuda T, Kohno Y, Horm Res 2000;53(3):157-60 6. A novel bioassay based on human growth hormone (hGH) receptor mediated cell proliferation: measurement of 20K-hGH and its modified forms. Ikeda M, Wada M, Takahashi S, Growth Horm IGF Res 2000 Oct;10(5):248-55.