Growth Hormone, Recombinant, Carp (GH1, GH, GHN, GH-N, hGH-N,Pituitary growth hormone, Growth hormone 1, Somatotropin)

Growth-Hormone is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature.\n\nSource: Growth Hormone Carp is a single, non-glycosylated, polypeptide chain containing 188aa having a molecular mass of 21.4kD.\n\nDimers and Aggregates:\n(same/less than) 1% as determined by silver-stained SDS-PAGE gel analysis. \n\nEndotoxin:\n(same/less than) 0.1ng/ug (IEU/ug) of rbrGH. \n\nProtein Content:\nProtein quantitation was carried out by two independent methods: \n1. UV spectroscopy at 280nm. \n2. Analysis by RP-HPLC, using a standard solution of GH as a Reference Standard.\n\nReconstitution:\nReconstitute the lyophilized rbrGH in sterile 18MOmega-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. \n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

G9000-08B

Size

100ug

Form

Supplied as a lyophilized powder from 0.3% sodium bicarbonate pH 8. Reconstitute with sterile dH2O, 0.1% BSA or HSA to 0.1-1mg/ml.

Purity

(same/more than) 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.

References

1. Growth hormone expression in ontogenic development in gilthead sea bream. Herrero-Turrion MJ, Rodriguez RE, Gonzalez-Sarmiento R, Cell Tissue Res 2003 Jul;313(1):81-91 2. Immunocytochemical and ultrastructural characterization of mammosomatotrope-, growth hormone-, and prolactin-cells from the gilthead sea bream (Sparus aurata l., Teleostei): an ontogenic study. Villaplana M, Garcia Ayala A, Agulleiro B, J Morphol 2003 Mar;255(3):347-57 3. Endocrine mediators of seasonal growth in gilthead sea bream (Sparus aurata): the growth hormone and somatolactin paradigm. Mingarro M, Vega-Rubin de Celis S, Pendon C, Gen Comp Endocrinol 2002 Sep;128(2):102-11 4. Identification of mammosomatotropes, growth hormone cells and prolactin cells in the pituitary gland of the gilthead sea bream (Sparus aurata L., Teleostei) using light immunocytochemical methods: an ontogenetic study. Villaplana M, Garcia Ayala A, Agulleiro B, Anat Embryol (Berl) 2000 Nov;202(5):421-9 5. Growth hormone as a function of age and dietary protein: energy ratio in a marine teleost, the gilthead sea bream (Sparus aurata). Marti-Palanca H, Martinez-Barbera JP, Valdivia MM, Growth Regul 1996 Dec;6(4):253-9 6. Ontogeny of growth hormone protein and mRNA in the gilthead sea bream Sparus aurata. Funkenstein B, Cohen I, Growth Regul 1996 Mar;6(1):16-21.