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Growth Hormone, Recombinant, Human (hGH)

Cat no: G9000-09

Growth Hormone, Recombinant, Human (hGH)

Human growth hormone (hGH, somatotropin), a protein hormone from the anterior lobe of the pituitary gland, comprises a single polypeptide chain of 191 amino acid residues. The growth hormone amino acid sequence varies considerably between species, and non-primate growth hormones have little activity in man. Its main function is the stimulation of somatic and bone growth, as well as an increase in the size and mass of organs and tissues.\n\nSequence (192aa):\nMFPTIPLSRL/ FDNAMLRAHR/ LHQLAFDTYQ/ EFEEAYIPKE/ QKYSFLQNPQ/ TSLCFSESIP/\nTPSNREETQQ/ KSNLELLRIS/ LLLIQSWLEP/ VQFLRSVFAN/ SLVYGASDSN/ VYDLLKDLEE/\nGIQTLMGRLE/ DGSPRTGQIF/ KQTYSKFDTN/ SHNDDALLKN/ YGLLYCFRKD/ MDKVETFLRI/\nVQCRSVEGSC/ GF\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G9000-09

Size

500ug

Form

Supplied as a liquid in 88mM mannitol, 5mM phosphate, pH 7.4.

Purity

Purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer ((same/more than) 95% by SDS PAGE). Endotoxin: (same/less than)1Eu/ug

References

1. Wickens, et al., J. Biol. Chem. 253(7): 2483-2495 (1978). 2. Goeddel, et al., Proc. Natl. Acad. Sci. 76: 106-110 (1979). 3. Chang, et al., Nature 275: 617-624 (1978). 4. Ullrich, et al., Science 196: 1313-1319 (1977).

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