Growth Hormone, Recombinant, Sheep (sGH)

A polypeptide that is secreted by the adenohypophysis. Growth hormone, also known as somatotropin, stimulates mitosis, cell differentiation and cell growth. Species-specific growth hormones have been synthesized. GH augments the cytolytic activity of T-cells, antibody synthesis, and granulocyte differentiation induced by GM-CSF. GH also enhances production of TNF-alpha, generation of superoxide anions from peritoneal macrophages, and natural killer activity. \n\nRecombinant Ovine Growth Hormone is a single, non-glycosylated polypeptide chain containing 200 amino acids and having a molecular mass of 22015 Dalton. \n\nSequence:\nThe sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Phe-Pro-Ala. \n\nDimers and Aggregates:\n(same/less than) 1% as determined by silver-stained SDS-PAGE gel analysis. \n\nBiological Activity:\nOvine Growth Hormone is fully biologically active when compared to standard. The activity as determined by the dose-dependent stimulation of the proliferation FDCP13B9 cells. \n\nEndotoxin:\n(same/less than) 0.1ng/ug (IEU/ug) of roGH. \n\nProtein Content:\nProtein quantitation was carried out by two independent methods: \n1. UV spectroscopy at 280nm using the absorbency value of 0.7 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). \n2. Analysis by RP-HPLC, using a calibrated solution of Ovine-GH as a Reference Standard.\n\nReconstitution:\nReconstitute the lyophilized Ovine-GH in sterile 18MOmega-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. \n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 0.4% NaHCO3 and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G9000-09K

Size

100ug

Form

Supplied as a lyophilized powder in 0.0045mM NaHCO3.

Purity

(same/more than) 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.

References

1. Optimization of immobilized metal ion affinity chromatography for single-step purification of recombinant ovine growth hormone expressed in Escherichia coli. Gupta V, Eshwari AN, Agarwal GP, J Chromatogr A 2003 May 23;998(1-2):93-101 2. The impact of a transgene for ovine growth hormone on the performance of two breeds of sheep. Adams NR, Briegel JR, J Anim Sci 2002 Sep;80(9):2325-33 3. Kinetics of inclusion body production in batch and high cell density fed-batch culture of Escherichia coli expressing ovine growth hormone. Panda AK, Khan RH, Totey SM, J Biotechnol 1999 Oct 8;75(2-3):161-72 4. Effect of the codon following the ATG start site on the expression of ovine growth hormone in Escherichia coli. Puri N, Appa Rao KB, Panda AK, Protein Expr Purif 1999 Nov;17(2):215-23 5. Production and characterisation of deletion mutants of ovine growth hormone. Sami AJ, Wallis OC, J Mol Endocrinol 1999 Aug;23(1):97-106 6. Identification of novel sites in the ovine growth hormone receptor involved in binding hormone and conferring species specificity. Allan GJ, Shand JH, Flint DJ, Eur J Biochem 1999 Apr;261(2):555-62.