GRP78 (78kD Glucose-regulated Protein, GRP-78, Endoplasmic Reticulum Lumenal Ca(2+)-binding Protein grp78, Heat Shock 70kD Protein 5, Immunoglobulin Heavy Chain-binding Protein, BiP, HSPA5)

GRP78, a 78kD glucose-regulated protein, also known as BiP or immunoglobulin heavy chain binding protein, is a stress-response protein which is induced by agents or conditions that adversely affect endoplasmic reticulum (ER) function. This protein is essential for the proper glycosylation, folding and assembly of many membrane bound and secreted proteins. GRP78 is critical for maintenance of cell homeostasis and the prevention of apoptosis. GRP78 protein levels have been shown to be a reliable biomarker of hypoglycermia as well as serving a neuroprotective function in neurons exposed to glutamate and oxidative stress. GRP78 levels are reduced in the brains of Alzheimer's Disease patients and decreased expression of GRP78 is found associated with missense mutations in the human presenilin-1 (PS1) gene. The induction of the GRP78 protein has been associated with the development of drug-resistance to antitumor drugs.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-3ug/ml\nImmunohistochemistry (formalin fixed paraffin embedded): 5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G3057-22W

Size

100ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

P Type

Pab

Purity

Purified by Protein G affinity Chromatography.

Isotype

IgG

References

1. Corrigall,V.M., Bodman-Smith,M.D., Brunst,M., Cornell,H. and Panayi,G.S., Inhibition of antigen-presenting cell function and stimulation of human peripheral blood mononuclear cells to express an antiinflammatory cytokine profile by the stress protein BiP: relevance to the treatment of inflammatory arthritis, Arthritis Rheum. 50 (4), 1164-1171 (2004). 2. Reddy,R.K., Mao,C., Baumeister,P., Austin,R.C., Kaufman,R.J. and Lee,A.S., Endoplasmic reticulum chaperone protein GRP78 protects cells from apoptosis induced by topoisomerase inhibitors: role of ATP binding site in suppression of caspase-7 activation, J. Biol. Chem. 278 (23), 20915-20924 (2003). 3. Watson,L.M., Chan,A.K., Berry,L.R., Li,J., Sood,S.K., Dickhout,J.G., Xu,L., Werstuck,G.H., Bajzar,L., Klamut,H.J. and Austin,R.C., Overexpression of the 78-kDa glucose-regulated protein/immunoglobulin-binding protein (GRP78/BiP) inhibits tissue factor procoagulant activity J. Biol. Chem. 278 (19), 17438-17447 (2003). 4. Shuda,M., Kondoh,N., Imazeki,N., Tanaka,K., Okada,T., Mori,K., Hada,A., Arai,M., Wakatsuki,T., Matsubara,O., Yamamoto,N. and Yamamoto,M., Activation of the ATF6, XBP1 and grp78 genes in human hepatocellular carcinoma: a possible involvement of the ER stress pathway in hepatocarcinogenesis, J. Hepatol. 38 (5), 605-614 (2003).

Additional Info

Recognizes human GRP78. Species Crossreactivity: mouse. Species sequence homology: rat, chimpanzee, canine, mcaque, zebrafish, xenopus.