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GRPEL1, aa28-217, Recombinant, Human (grpE Protein Homolog 1, Mitochondrial, HMGE, Mt-GrpE#1, GREPEL1)

Cat no: G3057-67

GRPEL1, aa28-217, Recombinant, Human (grpE Protein Homolog 1, Mitochondrial, HMGE, Mt-GrpE#1, GREPEL1)

GRPEL1 is an essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. This protein seems to control the nucleotide-dependent binding of mitochondrial HSP70 to substrate proteins.\n\nSource:\nRecombinant corresponding to aa28-217 of human GRPEL1 protein, fused to His-tag at N-terminus, expressed in E.coli.\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MCTATKQKNS GQNLEEDMGQ SEQKADPPAT EKTLLEEKVK LEEQLKETVE KYKRALADTE NLRQRSQKLV \nEEAKLYGIQA FCKDLLEVAD VLEKATQCVP KEEIKDDNPH LKNLYEGLVM TEVQIQKVFT KHGLLKLNPV GAKFDPYEHE ALFHTPVEGK \nEPGTVALVSK VGYKLHGRTL RPALVGVVKE A\n\nMolecular Weight: 23.6kD (211aa) confirmed by MALDI-TOF\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G3057-67

Size

50ug

Form

Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.2M sodium chloride, 1mM DTT, 10% glycerol.

Purity

~90% (SDS-PAGE).

References

1. Borges JC., et al. (2003) J Biol Chem. 278(37):35337-44. 2. H

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