GS28 (GOS28, p28)

GS28, also known as p28 or GOS28, is a 28kD integral membrane protein on the surface of the Golgi apparatus that serves as a t-SNARE in ER to Golgi transport (2). The N-terminal of GS28/GOS28 is exposed to the cytosol and is anchored to the cis Golgi via a 20 amino acid carboxyl-terminal hydrophobic tail (2). GS28 co-immunoprecipitates complexes consisting of syntaxin 5, rbet1, membrin, rsec22, and rsly1, and is therefore implicated in ER-to-Golgi or intra-Golgi vesicle transport (3). ATP hydrolysis by NSF in the presence of alpha-SNAP causes the dissociation of GOS28 and syntaxin 5 complex (3).\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot (ECL): 1:1000 \nOptimal dilutions to be determined by the researcher.\n\nPositive Controls:\nRat Brain Tissue Extract \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G9005-75

Size

50ug

Applications

WB

Hosts

Mouse

Reactivities

Mouse, Rat, Bov, Can, Ch/Bird, Prc, Rabbit, Sheep

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% azide, 50% glycerol.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Subramaniam, V. N., Krijnse-Locker, J., Tang, B.L., Ericsson, M., Yusoff, A.R., Griffiths, G. and Hong, W. (1995) J. Cell. Science 108: 2405-2414. 2. Subramaniam, V. N., Peter, F., Phlip, R., Wong, S.H. and Hong, W. (1996) Science 272: 1161-1163. 3. Hay, J., Chao, D.S., Kuo, C.S. and Scheller, R.H. (1997) Cell 89: 149-158. \n4. Espenshade, P.J., Li, W.P., Yabe, D. (2002) Proc Natl Acad Sci USA 99(18): 11694-11699.

Additional Info

Recognizes rat GS28 at~28kD. Recognized as a Cis Golgi marker, exposed to the cytosol, as determined by indirect immunofluoresence microscopy. Species Crossreactivity: mouse, hamster, rabbit, bovine, sheep, porcine, canine and chicken.