GTPase Activating Rap/RanGAP Domain-like 1 (GARNL1, DKFZp566D133, GRIPE, KIAA0884, TULIP1)

GARNL1 (GTPase activating Rap/Ran-GAP domain-like 1), contains one Rap-GAP domain. It is expressed during embryogenesis with E12. During development, GARNL1 expression decreases, persisting at high levels only in neurons of the adult brain. GARNL1 localizes to the cytoplasm where it may play a role regulating GTP hydrolysis of proteins such as Ran and Rap. GARNL1 is imported to the nucleus via dimerization with E12 and interacts with the HLH region of E12 and may function to negatively regulate the transcription of E12-dependent downstream target genes. This suggests that at least a portion of the function of GARNL1 is dependent upon its association with E12. GARNL1 may also associate with other HLH proteins and influence a variety of HLH signaling cascades. In adult brain, GARNL1 activity does not involve E12 and therefore it may serve a different function in developed neural tissue.\n\nApplications: \nSuitable for use in Western Blot and Dot Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G9006-40

Size

50ug

Applications

WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a sterile-filtered liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Nagase, T., et al. 1999. Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 5: 277-286. 2. Heng, J.I. and Tan, S.S. 2002. Cloning and characterization of GRIPE, a novel interacting partner of the transcription factor E.in developing mouse forebrain. J. Biol. Chem. 277: 43152-43159. 3. Online Mendelian Inheritance in Man, OMIMTM. 2002. Johns Hopkins University, Baltimore, MD. MIM Number: 608884. World Wide Web URL: http://www.ncbi.nlm.nih.gov/omim/ 4. Schwarzbraun, T., et al. 2004. Cloning, genomic structure, and expression profiles of TULIP1 (GARNL1), a brain-expressed candidate gene for 14q13- linked neurological phenotypes, and its murine homologue. Genomics 84: 577-586. 5. Chen, C.F., et al. 2007. Laying traits and underlying transcripts, expressed in the hypothalamus and pituitary gland, that were associated with egg production variability in chickens. Theriogenology 68: 1305-1315.

Additional Info

Recognizes human GTPase Activating Rap/RanGAP Domain-like 1.