Guanidinoacetate N-methyltransferase isoform, Recombinant Human, aa1-256, His-Tag (GAMT)

Supplier:

United States Biological

Cat no: G9028

Guanidinoacetate N-methyltransferase isoform, Recombinant Human, aa1-256, His-Tag (GAMT)

Guanidinoacetate N-methyltransferase isoform a, also known as GAMT is a methyltansferase that converts guanidoacetate to creatine, using S-adenosylmethionine as the methyl domor. This enzyme participates in the two-step synthesis of the compound creatine from the protein building blocks glycine, arginine, and methionine. It is involved in providing energy for muscle contraction, and is also important in nervous system functioning. Also GAMT is active in the liver, pancreas, and kidneys. Recombinant human GAMT, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.\n\nSource:\nRecombinant corresponding to human GAMT, his-tagged, expressed in E. coli. \n\nAA sequence:\nMGSSHHHHHH SSGLVPRGSH MSAPSATPIF APGENCSPAW GAAPAAYDAA DTHLRILGKP VMERWETPYM HALAAAASSK GGRVLEVGFG\nMAIAASKVQE APIDEHWIIE CNDGVFQRLR DWAPRQTHKV IPLKGLWEDV APTLPDGHFD GILYDTYPLS EETWHTHQFN FIKNHAFRLL\nKPGGVLTYCN LTSWGELMKS KYSDITIMFE ETQVPALLEA GFRRENIRTE VMALVPPADC RYYAFPQMIT PLVTKG\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and freeze at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.

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SPECIFICATIONS

Catalog Number

G9028

Size

100ug

Reactivities

Hum

Form

Supplied as a liquid in 20mM Tris-HCl, pH8.0, 1mM DTT, 10% glycerol.

Purity

~95% (SDS-PAGE) Purified by immunoaffinity chromatography.

References

1.Almeida LS., et al. (2007) Mol Genet Metab. 91(1):1-6. \n2.Jenne DE., et al. (1997) Biochem Biophys Res Commun. 238(3):723-7. \n

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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