Guanine Neculeotide Exchange Factor H1 (GEF-H1, Rho/Rac guanine nucleotide exchange factor 2, Proliferating cell nucleolar antigen p40, Lfc)

GEF-H1 can activate the small GTPase RhoA, but not Rac1 or Cdc42. Rho family GTPases are central regulators of the cytoskeleton. GEF-H1 can associate with different cytoskeletal structures, namely microtubules and the actin cytoskeleton. It has also been proposed to mediate crosstalk between the two types of filaments. Localization of GEF-H1 differs between cell types. In MRC-5 fibroblast cells, GEF-H1 localizes to stress fibers. In epithelial cells, GEF-H1 is associated with apical tight junctions and involved in regulating paracellular permeability of small hydrophilic tracers. Furthermore, its subcellular localization changes in mitotic cells, where endogenous GEF-H1 is concentrated at mitotic spindles. GEF-H1 is capable of binding to the F-actin binding junctional adaptor cingulin. Binding to cingulin inhibits GEF-H1 and results in the downregulation of RhoA and inhibition of G1/S phase transition. In low confluent cultured cells, the localization of GEF-H1 is predominantly cytoplasmic. With increasing density of the cells, free GEF-H1 is sequestered at the tight junctions by cingulin.\n\nApplications: \nSuitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence: 1:50 \nELISA: 1:50\nImmunoprecipitation: 1:50\nImmunohistochemistry: frozen\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G9039-54

Size

100ug

Applications

ELISA, IF, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, Can

Form

Supplied as a liquid in PBS, pH 7.2, 1mg/m l0.1% BSA, 0.02% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Benais-Pont, G et al; Identification of a tight junction-associated guanine nucleotide exchange factor \nthat activates Rho and regulates paracellular permeability. J Cell Biol 2003, 160: 729 2. Aijaz, S et al; Binding of GEF-H1 to the tight-junction-associated adaptor cingulin results in inhibition of Rho signaling and G1/S phase transition. Dev Cell 2005, 8: 777.

Additional Info

Recognizes canine guanine nucleotide exchange factor H1 (GEF-H1). Species Crossreactivity: human.

Alternative Names

Rho/Rac Guanine Nucleotide Exchange Factor 2, ARHGEF2, GEF, GEFH1