Guanine Nucleotide Dissociation Inhibitor (GDI)

Small molecular weight GTP-binding proteins of the Rab family have been shown to play an essential role in the regulation of vesicular trafficking. The cycling of these proteins between the inactive (GDP-bound) and active (GTP-bound) forms is thought to catalyze docking to specific target membranes. To date, several accessory proteins which serve to regulate the association of Rab proteins with GTP or GDP have been identified(4,5,6). The best characterized of these Rab accessory proteins is Rab3A GDI(4,5,7,8). Rab3A GDI forms cytosolic complexes with Rab3A and, in vitro, promotes the dissociation of Rab3A from membranes(7,8). Two other isoforms of GDI have recently been isolated and have been termed GDI b and GDI-2, respectively(3,5). Studies have revealed that the proportion of Rab proteins found associated with a particular GDI isoform depends upon the abundance of the specific GDI in the cytosol, as well as on the tissue and cell type examined(5,6,7).\n\nApplications: \nSuitable for use in ELISA, Western Blotting, Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.5

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SPECIFICATIONS

Catalog Number

G9039-50

Size

100ug

Applications

ELISA, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov

Form

Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Wilson, A.L., et al; Mol. Biol. Cell; (Submitted) (1995).\n2. Pfeffer, S.R., et al; Trends Cell Biol.:2: 41 (1992) (Review Article).\n3. Takai, Y., et al; Int Rev. Cytol.:133: 187 (1992) (Review Article).\n4. Regazzi, R., et al; J. Biol. Chem. 267:17512-17519 (1992).\n5. Sasaki, T., et al; J. Biol. Chem. 256:2333-2337 (1990).\n6. Yang, C., et al; J. Biol. Chem. 269: 31891-31899 (1994).\n7. Nishimura, N., et al;. J. Biol. Chem. 269:14191-14198 (1994).\n8. Ullrich, O., et al;. J. Biol. Chem. 268:18143-18150 (1993).\n9. Shisheva, A., et al; Mol. Cell. Biol. 14: 3459-3468 (1994).

Additional Info

Speci?c for GDI-1 (~50kD). GDI-1 is also referred to as GDI-a and Rab3A GDI (1) . Crossreactivity to GDI-2 (~46kD) has been observed. A synthetic peptide, corresponding to a 20 amino acid sequence of bovine brain Rab Guanine Nucleotide Dissociation Inhibitor (GDI), was conjugated to carrier protein and used as the immunogen. This sequence is conserved in both mouse and rat GDI proteins. Human, mouse and rat. Reactivity has been con?rmed by Western blot analysis of extracts derived from human HEK293 cells and of recombinant bovine Rab-GDI.