Guanylate Binding Protein-1, Human (Guanine Nucleotide Binding Protein 1, GTP Binding Protein 1, GBP1, GBP-1, HuGBP-1, Interferon-induced Guanylate Binding Protein 1) BioAssay(TM) ELISA Kit

Human guanylate binding protein-1 (hGBP-1) is an interferon-=-induced GTPase with a molecular weight of 67kDa which places it in the large GTPase family (1). hGBP-1 hydrolyzes GTP and GDP but fails to hydrolyze ATP, CTP, or UTP at a reasonable rate (1). GTPases induced by interferons, such as hGBP-1, play a significant role in the immune response to microbial and viral pathogens. Moreover, hGBP-1 regulates the inhibitory effects of inflammatory cytokines on endothelial cell proliferation, migration and invasiveness (2,3,4,5) and has been shown to be associated with the inhibition of angiogenesis and a highly significantly increased survival of patients with colorectal carcinoma (6). Furthermore, it has been shown that hGBP-1 is\nsecreted from endothelial cells and is the first GTPase known to be secreted (7). hGBP-1 exhibits anti-viral activity against vesicular stomatitis virus and encephalomyocarditis and has been shown to be detectable in the cerebrospinal fluid of patients with bacterial meningitis (7,8).\n\nSample Type:\nSerum, CSF\n\nKit Components (Enough for 1x96-well plate):\nWash solution concentrate (20X), 1x20ml\nDiluent buffer concentrate (5X), 1x25ml\nDetection antibody, 1x10ul, polyclonal rabbit anti-GBP-1 antibody.\nDetection enzyme, 1x10ul, Goat anti-Rabbit IgG\nTMB substrate solution A, 1x6ml\nTMB substrate solution B, 1x6ml\nStop solution, 1x12ml, 0.3M sulfuric acid\nMicroteter plate, coated with rat anti-human GBP-1, 1x 1plate \nRecombinant GBP-1 calibrator, 1x40ul\n\nStorage and Stability:\nStore all components at 4 degrees C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

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