HAND2 (dHAND, Thing-2, Hed, HAND Family Transcription Factor)

HAND2 (also known as dHAND, Thing-2 and Hed) is a 24kD, class B member of the basic helix-loop-helix transcription factors superfamily. HAND2 is essential for cardiac morphogenesis and required for vascular development and regulation of angiogenesis, possibly through VEGF signaling. HAND2 is also implicated in limb development acting as an upstream regulator of sonic hedgehog. Alternative initiation and internal deletions result in at least three potential HAND2 isoforms. Human and mouse HAND2 proteins are 100% identical.\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.5-1.0ug/ml. The detection limit for rhHAND2 and rmHAND2 is approximately 1.3ng/well. \nWestern Blot: 0.1-0.2ug/ml. The detection limit for rhHAND2 and rmHAND2 is approximately 25ng/lane under non-reducing and reducing conditions.\nImmunohistochemistry: 10ug/ml to detect HAND2 in mouse embryos. Cells were fixed with PBS containing 4% paraformaldehyde and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA. After blocking, cells were incubated with diluted primary antibody followed by NorthernLightsTM 557-coupled anti-goat IgG at RT in the dark. Between each step, cells were washed with PBS containing BSA. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute by adding sterile 40-50% glycerol, PBS. Aliquot and store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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