HAPLN2 (Hyaluronan and Proteoglycan Link Protein 2, Brain Link Protein 1, BRAL1)

BRAL1 is a member a superfamily consisting of several highly homologous hyaluronan and proteoglycan binding link proteins. BRAL1 is predominantly expressed in brain tissue and spinal cord. Like other members in the link-module superfamily, BRAL1 contains an immunoglobulin-like fold and two proteoglycan tandem repeats (PTRs). Its mRNA expression pattern is similar to other lectican proteoglycans, suggesting that BRAL1 may act to stabilize the binding between the extracellular matrix molecule hyaluronan and brevican. Immunostaining of mouse brain showed BRAL1 expression at P20 in the white matter of the developing cerebellum and in myelinated fiber tracts in the adult brain, suggesting that expression starts when axonal myelination occurs.\n\nApplications:\nSuitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml\nImmunofluorescence: 20ug/ml\nImmunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHuman Brain Tissue Lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

146240

Size

100ug

Applications

ELISA, IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Spicer AP, Joo A, and Bowling Jr. RA. A hyaluronan binding link protein gene family whose members are physically linked adjacent to chrondroitin sulfate proteoglycan core protein genes. J. Biol. Chem. 2003; 278:21083-91. 2. Hirakawa S, Oohashi T, Su W-D, et al. The brain link protein-1 (BRAL1): cDNA cloning, genomic structure, and characterization as a novel link protein expressed in adult brain. Biochem. Biophys. Res. Comm. 2000; 276:982-9. 3. Oohashi T, Hirakawa S, Bekku Y, et al. Bral1, a brain-specific link protein, colocalizing with the versican V2 isoform at the nodes of Ranvier in developing and adult mouse central nervous systems. Mol. Cell Neurosci. 2002; 19:43-57.

Additional Info

Recognizes human BRAL1. Species Crossreactivity: mouse and rat.